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R6K

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“It’s a great system that can be recommended to any lab that cares about their protein solutions"
Patrick Celie – NKI

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ScreenTape R6K has been specifically developed for efficient and reproducible RNA characterisation and quality control. This product will significantly simplify your routine RNA QC procedures, making them faster and more reliable. No complex chip priming is required, and samples are run in separate channels to prevent sample carryover. The ScreenTape Degradation Value (SDV) standardises RNA quality control for eukaryotic samples by providing an objective and intuitive measure of RNA degradation. Depending on your application, ScreenTape R6K can be used with three different loading buffers: Standard , High Sensitivity or cRNA. For fragment sizing we recommend using the Norgen Biotek RNA ladder with our RNA products (Catalogue number 15006), which can be ordered here.

Click here to download datasheet.

Key features:

Sample to answer is achieved in one minute per sample
SDV (ScreenTape Degradation Value) provides accurate and objective total RNA degradation assessment
No chip or device priming – R6K is fully pre-packaged yielding excellent reproducibility
Cross priming and lane to lane contamination are avoided due to parallel processing
RNA profiles are easy to interpret and annotate
ScreenTape can be used more than once – there is no need to batch samples as spare lanes can always be used at a later date
Three different loading buffers give you flexibility in your RNA applications: Standard (RNA concentrations of 5 to 100 ng/µl), High Sensitivity (RNA concentrations of 0.1 to 5 ng/µl) and cRNA (between 100 and 1000 ng/µl)

Total RNA analysis

cRNA analysis

RNA samples

Total RNA, cRNA, mRNA, fragmented cRNA

Qualitative range

0.5 - 100 ng/µl total RNA

Limit of detection 100 pg/µl

Sample volume loaded

2 µl

Analysis time per sample

One minute

Number of lanes / tape

RNA anaylsis values

SDV for total RNA,

28S/18S ratio, peak position, RNA quantity

What is the ScreenTape Degradation Value (SDV)?

The SDV is derived from a mathematical model that calculates an objective and quantitative measurement of RNA degradation. When separated by electrophoresis, eukaryotic total RNA samples display three major peaks representing small RNAs, 18S rRNA and 28S rRNA. However, underlying these dominant profile features are low levels of a complex population of RNAs that cover a wide spectrum of molecular weights. As total RNA degrades, the 28S and 18S peaks slowly disappear while peaks from degraded material emerge between the 18S and small RNAs. The SDV represents the ratio of the average degradation peak signal to the 18S peak signal multiplied by 100. The higher the SDV the higher the level of RNA degradation. The SDV is independent of peak selection boundaries and unaffected by sample concentration. It can therefore facilitate eukaryotic RNA QC analysis in a more objective and reproducible manner than other currently available automated methods.

Below are two typical screen grabs from an R6K analysis, showing automated 18S and 28S peak recognition, as well as SDV values calculated for each RNA sample. Results are easy to interpret and annotate and can be presented in a GLP compliant report.

Figure 1: ScreenTape R6K analysis of total RNA from HepG2 cells. This is a typical Lab901-GeneTools screen grab showing the automated analysis achieved by R6K ScreenTape. Lane 1 shows an RNA ladder with fragments at 4kb, 2kb, 800b, 600b, 400b, 200b and 50b. Lanes 2 to 8 contain total RNA from HepG2 cells that have been heat degraded at 90ºC for 0, 3, 6, 9, 12, 15 and 21 minutes respectively. The results show a gel image and electropherogram for each sample where the 28S and 18S peaks are automatically indentified. SDVs are displayed in the table and are colour coded (green: good RNA, yellow: average RNA and red: poor RNA). The boundaries for these values can be set by the user. All lanes contain a 50b bottom marker, which is a standard component of the R6K loading buffer.

Figure 2: The Lab901-GeneTools software allows you to overlay electropherograms for easy sample comparison. This screen grab shows three overlaid traces from different preparations of HepG2 total RNA. The green electropherogram is from a good RNA sample with an SDV = 3, the black trace is from an average RNA sample with an SDV = 13.8 and the red trace is from a poor RNA sample with an SDV = 67.2.