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|  |  | ScreenTape P200 is a specialised product for the electrophoresis of proteins ranging from 10 to 200 kDa. Up to 16 protein samples can be analysed reliably in less than one minute per sample, without any manual intervention. The results are automatically presented by the integrated Lab901-GeneTools analysis software, which identifies and sizes protein peaks, calculates product purity and compares different protein profiles. Protein expression and purification methods can now be streamlined with this new automated SDS PAGE product. Click here to download datasheet. Key features: - P200 reagents are all pre-packaged providing accurate and reproducible electrophoresis results with no need to handle hazardous chemicals
- Sample-to-answer is achieved in less than 1 minute per sample – the protein analysis bottleneck is eliminated and purification methods are streamlined
- Full automation provides a true walk-away process that eliminates all the complicated and subjective staining and destaining methods involved in standard SDS-PAGE
- ScreenTape can be used more than once - there is no need to batch samples as spare lanes can be used at a later date
- Regulatory compliance is attained with a GLP standard print-out that includes any post-analysis modifications
|  | | Protein A/G purification His-Tag purification Monitoring of fermentation process QC before MS/crystallography MAb stability testing Monoclonal Antibody QC Purification monitoring Monitoring of tag removal from fusion protein |  |
| | Sizing range | 10 to 200 kDa |
| MW accuracy (average
deviation) | < 10% |
| | MW precision | < 2% CV |
| Suggested sample
concentration | 100 to 1000 µg/ml |
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| | Quantitative range | 5 - 5000 µg/ml |
| | Sample volume needed | 2 µL |
| | Number of analyses per ScreenTape | 2 to 16 |
| | Typical sensitvity | 5 µg/ml |
| | | | Figures 1 and 2 below are two screen grabs of protein samples run on P200, showing the top and bottom inlane standards in red and the sample bands in blue. 
Figure 1: Lab901–GeneTools screen grab of seventeen ion exchange fractions. Protein analysis results are automatically presented by the ScreenTape P200 platform, without the need for a manual gel documentation system. All electrophoresis results are presented as a gel image. When a lane is selected it is displayed as an electropherogram (lane 5 has been selected in this view) where protein peaks are annotated with molecular weight (shown here) or quantity. All results are also displayed in a table containing data such as peak number, molecular weight, quantity and % purity. Lane 1 contains a molecular weight standard with proteins at 200, 120, 85, 60, 50, 30, 20 and 10 kDa. 
Figure 2: Screen grab from Lab901-GeneTools software the overlay of eight protein profiles from samples containing bands at 120, 85, 60, 50, 30 and 20 kDa. The near perfect overlap of the peaks demonstrates the excellent reproducibility of the P200 platform for protein applications. |
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